Mr. Lara is a career scientific and commercial photographer and professor of information systems and internet technologies.
For Corporate/Commercial Photography call or fill out the request form here
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Direct 904-302-6025
Scientific & Product Photography
What is macro?
Photomacrography is usually defined as any magnification at the film plane of 1X (life size) and higher. Anything below life size is considered a “closeup.” Simple diopter lenses placed in front of the normal camera lens, or sometimes a small extension tube between the camera and main lens, usually result in magnifications below a 1:1 ratio.
Macro photography is close-up photography; the classical definition is that the image projected on the "film plane" (i.e., film or a digital sensor) is close to the same size as the subject. On 35 mm film (for example), the lens is typically optimized to focus sharply on a small area approaching the size of the film frame. Most 35mm format macro lenses achieve at least 1:2, that is to say, the image on the film is 1/2 the size of the object being photographed. Many 35mm macro lenses are 1:1, meaning the image on the film is the same size as the object being photographed.

| Sacsayhuamán from the Quechua composed it comes Sacsay which means fullfill and huamán the hawk. The hawk, bird of prey >>> | Chinchero These series of images is the result of a trip to Ollanta in a Sunday afternoon slide show >>> |
Arequipa, also known as the Ciudad Blanca. Arequipa (2,350 m.a.s.l.) slide show >>> |
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Click the link to view images of the Amazonas. A view of the rain forest near the Nanay river >> start the show |
Here is an aerial view of the "Colibri", one of the many Nazca lines embedded >>. start the slide show. |
This shot is taken of the Ollaytantambo ruins. This espectcular site is oneof my favorite stops when I visit Cuzco>> start the slide show. |
What is micro?
Photomicroscopy is the process of documenting images on film as seen through a microscope. It is usually defined as any magnification at the film plane of 10X (life size) and higher.
Transillumination considerations:
Glare
The intermediate plane aperture should be stopped down to reduce glare as well as bring out the detail in the image
Numerical Aperture
In simple words, the higher the NA, the larger the cone of light accepted by the lens, the higher its resolution, and the lower its depth of field. Most microscope lens have no iris diaphragm like a camera lens and so are used at full aperture -- but with microscopes one is generally interested in resolution, not in depth of field. A 100 X oil immersion lens typically has a NA of 1.24 to 1.4, which corresponds to a limiting resolution of about 0.2 micrometers with the proper illumination. For comparison, a typical mammalian cell is 8 to 10 micrometers in diameter. As a camera lens is stopped down, its NA and resolution fall and the depth of field increases.
Optics - use lenses of a higher numerical aperture if there is not enough detail in the image and to get the best resolution.
Fluorescence microscopy, use the highest numerical aperture possible for the magnification desired, especially if the staining is dim.
Color - increase the luminances filament light source to (3200 K) and place a blue filter to match 5500 K. Alternateviley use a xenon arc lamp which gives white light and provides a full color spectrum.For H&E staining (hemotoxylin/eosin), use of a didymium filter (BG20)to enhace the reds.
Black & White - To increase contrast use a very narrow band green filter should be placed in the light path.

Here is my son Pat riding his scooter, March 20, 2009
My hobbies include fast-motion photography, water-color painting, swimming, and of course my favorite sport, tennis. As a USTA member I travel the US to see the pros and have fun playing on clay or hard court surfaces. Got a racket?
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